Prevalence of CYP2C19 681G>A and 636G>A Gene Polymorphisms in Javanese Farmers Exposed to Chlorpyrifos

Authors

  • Jen Fuk Liem Department of Occupational Health and Safety, Faculty of Medicine and Health Science, Universitas Kristen Krida Wacana
  • Muchtaruddin Mansyur Community Medicine Department, Faculty of Medicine Universitas Indonesia
  • Safarina G. Malik Eijkman Institute for Molecular Biology, National Research and Innovation Agency
  • Dwi A. Suryandari Department of Biology, Faculty of Medicine Universitas Indonesia
  • Dewi S. Soemarko Community Medicine Department, Faculty of Medicine Universitas Indonesia
  • Imam Subekti Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia and Dr. Cipto Mangunkusumo General Hospital
  • Franciscus D. Suyatna Department of Pharmacology and Therapeutics, Faculty of Medicine Universitas Indonesia
  • Aria Kekalih Community Medicine Department, Faculty of Medicine Universitas Indonesia
  • Bertha Pangaribuan Prodia Occupational Health Institute International

DOI:

https://doi.org/10.47895/amp.v56i1.4498

Keywords:

CYP2C19, farmer, pesticide, susceptibility biomarker

Abstract

Objective. The objective of our study was to determine the genotype frequencies of CYP2C19*2 and *3 gene polymorphisms among Javanese farmers exposed to chlorpyrifos (CPF) in Central Java, Indonesia.

Methods. This cross-sectional study was conducted from July to October 2020 in Central Java, Indonesia, involving 151 vegetable farmers aged 18-65 years who used CPF for at least one year. CYP2C19*2 and *3 gene polymorphisms were analyzed using PCR-RFLP. Direct calculations were applied to calculate allele and genotype frequencies. The difference in genotype frequencies among the sex and cumulative exposure level (CEL) group was performed using the Chi-square test. In contrast, the proportion difference of allele frequencies was analyzed using Z-test.

Results. The frequency of CYP2C19 genotypes *1/*1, *1/*3, *1/*2, and *2/*2 were 64 %, 7 %, 23 % and 6 %. We observed no significant difference in the genotype distribution according to sex group and CEL group.

Conclusion. In summary, the prevalence of toxicologically relevant CYP2C19 polymorphisms was determined in the Javanese agricultural population. The CYP2C19 genotype may be helpful as an essential biomarker of genetic susceptibility towards CPF exposure. Nevertheless, further studies to confirm the role of CYP2C19 in this context are still needed.

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Published

2022-01-31

How to Cite

1.
Prevalence of CYP2C19 681G>A and 636G>A Gene Polymorphisms in Javanese Farmers Exposed to Chlorpyrifos. Acta Med Philipp [Internet]. 2022 Jan. 31 [cited 2025 Apr. 5];56(1). Available from: https://actamedicaphilippina.upm.edu.ph/index.php/acta/article/view/4498

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