Determination of the Antiangiogenic Activity of Telescopium telescopium (horn snail) Extract using in ovo Chorioallantoic Membrane (CAM) Assay

Abstract

Objective. To determine the antiangiogenic activity of Telescopium telescopium (Horn snail) extract using in ovo chorioallantoic membrane (CAM) assay.

Methods. Methanolic extract of Telescopium telescopium was subjected to modified Kupchan partitioning. Four treatment groups – negative control, positive control (quercetin), test samples, and blanks – were used for the in ovo chorioallantoic membrane (CAM) assay. ImageJ software was used to measure average vessel diameter (DV) and total length (LT) to determine the degree of vascularization, percent inhibition, and antiangiogenic activity. Biochemical screening was done for the crude extract and the fraction with the highest percent inhibition.

Results. Butanol fraction showed the highest percent inhibition in both average vessel diameter (DV) (417.30% ± 300.83) and total length (L T) (44.21% ± 8.11). There was no significant difference in the antiangiogenic activity of both crude and butanol fraction compared with quercetin. Biochemical screening confirmed the presence of sterols and carbohydrates in both fractions.

Conclusion. Crude extract and butanol fraction resulted in positive percent inhibition values, indicating inhibition of angiogenesis. They were found to have no significant difference with quercetin in regard to their antiangiogenic activity. Sterols were assumed as the biochemical class of the antiangiogenic compound of interest.